To apply BioPur first determine the nature of the problem:

1. Assess that the problem involves hydrocarbon/organic pollutants or contaminants.

(Check what BioPur degrades. Note BioPur will not degrade non-hydrocarbons/non-organics.)

2. Assess if problem is excessive BOD (bio-chemical oxygen demand) in wastewater in the system.
3. Assess if the problem is excessive FOG (fat, oil, grease) buildup in wastewater in the system.
4. Determine if the pollutant/contaminant is pure oil or organic waste deposited outside a system.

There are three different situations in which BioPur then has to be applied:

5. For open surfaces such as interior floors, concrete slabs, open land, and water.
6. For interior components of wastewater systems directly accessible such as grease traps, cisterns, holding tanks, septic tanks, and STPs.
7. For interior components of wastewater systems not directly accessible such as pipes.

For all three situations: the first steps for application of BioPur are:

1. Decide which type of BioPur is best to use. BioPur (Brown) is most effective for problems with excessive BOD, and BioPur (Blue) is best for problems of FOG buildup.
2. Determine the dilution ratio. It is best to start with a ratio of 5–10 parts water / 1 part concentrated BioPur for FOG and 10–15 parts / 1 part for other problems.
3. Dilute the concentrated BioPur with water and stir thoroughly until white foam appears (enzyme activation).
4. Wear appropriate personal protective equipment, including goggles.

For situation (1): Ensure BioPur covers the entire contaminated area. Using a sprayer is essential.

For situation (2): Apply BioPur to all visible FOG (use sprayer if available). If a sprayer is not available, pour diluted BioPur into the system upflow and close to the FOG buildup. Break large floating FOG masses into smaller pieces for faster degradation.

For situation (3): Pour diluted BioPur into drains, sinks, showers, or toilets that lead to the affected pipes. Apply in repeated small doses (250 ml) instead of one large dose.

After applying BioPur, monitor results, observe for reduction of FOG, sheen, or odor. Adjust dilution ratio and frequency based on results and site conditions.

Other important considerations about using BioPur: When BioPur is sprayed or poured into a wastewater system to address one problem, it will continue to flow through the system, benefiting other areas with excessive BOD or FOG. BioPur remains active in cisterns, holding tanks, septic tanks, and STPs for some time.